recombinant aav vectors raav  (ATCC)

Journal: Experimental and Therapeutic Medicine

Article Title: Increased temperature-related adeno-associated virus vectors transduction of ovarian cancer cells - essential signatures of AAV receptor and heat shock proteins

doi: 10.3892/etm.2019.8112

Figure Lengend Snippet: Transduction efficiency of AAV-293, Caov-3 and NIH:OVCAR-3 cells. (A) AAV-293, (B) Caov-3 and (C) NIH:OVCAR-3 cells were transduced with the rAAV/DJ vector under normal (37°C) and hyperthermic conditions (40 and 43°C) for 3 h. The green fluorescent protein positive cells were analyzed using (a) the Countess II FL Automated Cell Counter, (b) qPCR and visualized with an inverted fluorescence microscope under (c) bright field and (d) FITC (magnification, ×10; scale bar, 100 µm). Results are presented as the mean ± standard deviation. *P<0.05, **P<0.01 and ****P<0.0001 vs. 37°C. rAAV/DJ, recombinant adeno-associated viral hybrid serotype DJ; qPCR, quantitative PCR.

Article Snippet: To transduce the ovarian cancer cells, the recombinant AAV vector rAAV/DJ-cmv-eGFP (cat. no: 7101; Vector Biolabs) was added to the new, heated media with the multiplicity of infection (MOI) of 4×10 4 genome copies or 0 genome copies (non-transduction control group).

Techniques: Transduction, Plasmid Preparation, Fluorescence, Microscopy, Standard Deviation, Recombinant, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Increased temperature-related adeno-associated virus vectors transduction of ovarian cancer cells - essential signatures of AAV receptor and heat shock proteins

doi: 10.3892/etm.2019.8112

Figure Lengend Snippet: AAV-293, Caov-3 and NIH:OVCAR-3 cell miRNA analysis at normal temperatures. The selected miRNA responsible for silencing the cell transmission genes of rAAV (AAVR, HSPG1 and HSPG2) were assessed via reverse transcription-quantitative PCR. (A) Heat map represents the 2 −ΔCq data obtained from the studied miRNA. The distance clustering of the examined cells was elucidated. (B) Mean value of all analyzed miRNAs (2 −ΔCq ) characteristic of AAV-293, Caov-3 and NIH:OVCAR-3 cell lines. Mean values were calculated to better visualize the miRNA expression differences between studied cell lines. Data are presented as the mean ± standard deviation. *P<0.05 as indicated. miRNA, microcRNA; rAAV, recombinant adeno-associated viruses; AAVR, adeno-associated virus receptor; HSPG, heparin sulfate proteoglycan;

Article Snippet: To transduce the ovarian cancer cells, the recombinant AAV vector rAAV/DJ-cmv-eGFP (cat. no: 7101; Vector Biolabs) was added to the new, heated media with the multiplicity of infection (MOI) of 4×10 4 genome copies or 0 genome copies (non-transduction control group).

Techniques: Transmission Assay, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation, Recombinant

Journal: Experimental and Therapeutic Medicine

Article Title: Increased temperature-related adeno-associated virus vectors transduction of ovarian cancer cells - essential signatures of AAV receptor and heat shock proteins

doi: 10.3892/etm.2019.8112

Figure Lengend Snippet: Adhesion of ovarian cancer cell lines (Caov-3 and NIH:OVCAR-3) and AAV-293 under hyperthermic conditions. Plates were coated with laminin. Statistically significant differences were observed between cells transduced at 37°C and hyperthermia conditions and similarly between control cells inubated at 37°C and cells exposed to higer temperatures. Data are presented as the mean ± standard deviation. ○ P<0.05 vs. 37°C rAAV/DJ NIH:OVCAR-3; ○○○ P<0.001 vs. 37°C rAAV/DJ Caov-3; *P<0.05 vs. 37°C rAAV/DJ AAV-293 or Caov-3; ***P<0.001 vs. 37°C rAAV/DJ NIH:OVCAR-3; ****P<0.0001 vs. 37°C rAAV/DJ Caov-3 or NIH:OVCAR-3; ++ P<0.01 vs. 37°C control Caov-3; # P<0.05 vs. 37°C control Caov-3 or NIH:OVCAR-3; ### P<0.001 vs. 37°C control AAV-293; #### P<0.0001 vs. 37°C control NIH:OVCAR-3.

Article Snippet: To transduce the ovarian cancer cells, the recombinant AAV vector rAAV/DJ-cmv-eGFP (cat. no: 7101; Vector Biolabs) was added to the new, heated media with the multiplicity of infection (MOI) of 4×10 4 genome copies or 0 genome copies (non-transduction control group).

Techniques: Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Increased temperature-related adeno-associated virus vectors transduction of ovarian cancer cells - essential signatures of AAV receptor and heat shock proteins

doi: 10.3892/etm.2019.8112

Figure Lengend Snippet: Caov-3, NIH:OVCAR-3 and AAV-293 cell invasion assays under hyperthermic conditions. (A) transduced green fluorescent protein + cells observed on the lower Matrigel chamber after 24 h of incubation (FITC). (B) Fixed and stained (0.5% crystal violet) cells on the lower Matrigel chamber after 48 h of incubation (magnification, ×10; scale bars, 100 µm). (C) Number of migrating cells. Data are presented as the mean ± standard deviation. rAAV/DJ, recombinant adeno-associated viral hybrid serotype DJ.

Article Snippet: To transduce the ovarian cancer cells, the recombinant AAV vector rAAV/DJ-cmv-eGFP (cat. no: 7101; Vector Biolabs) was added to the new, heated media with the multiplicity of infection (MOI) of 4×10 4 genome copies or 0 genome copies (non-transduction control group).

Techniques: Incubation, Staining, Standard Deviation, Recombinant